LaboratoryJapanese

Seminar room / Desks

Seminar room

Weekly meetings and Progress meetings will be held.

Desks

Deskwork during research.

The area for molecular biology works

The area for molecular biology works

Western blotting (semi-dry or wet tank transfer), gel electrophoresis, or molecular works using E. coli.

Plasma generator

Adhesion improves after treating a material to which cells are difficult to attach.

NanoDrop

Used for DNA and RNA quantitation with only 1 µL of sample.

Inverted fluorescence microscope (Keyence, BZ-X710)

Images can be acquired quickly and easily

The area for immunocytochemistry

The area for immunocytochemistry

The area for molecular biology works: Western blotting (semi-dry or wet tank transfer), gel electrophoresis, or molecular works using E. coli.

Left:FUSION Solo S

Used for taking chemiluminescence images. Auto exposure, manual set of exposure time, and incremental exposure mode are useful.

Right:BIORUPTOR II

Sonicator in sealed box with circulating water cooler. It can sonicate 12 tubes at the same time.

Upper:BIO-RAD iMark microplate reader

used for protein assay and ELISA.

Bottom:Progmega GloMax multimode plate reader

sometimes used for mycoplasma tests.BIO-RAD thermal cyclers.

Gel electrophoresis and Gel Doc EZ Imager

The area for RNA experiments

The area for RNA experiments

Mainly used for RNA extraction and cDNA synthesis. Four block heaters and StepOnePlus PCR system.

QIAcube

Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits. It can purify max 12 samples at the same time.

Automated liquid handling system

mainly used for 384-well plate preparation for qRT-PCR.

QuantStudio 7 Real-Time PCR System

Installed in 2017. It supports 384-well format and TaqMan array.

Multi gas piping

The autochanger switches prevent the incubator from running out of the gas. N2 gas is for hypoxic culture.

In front of the culture room

In front of the culture room

Three liquid nitrogen tanks and one -150 degrees Celsius deep freezer are placed, and a total of 38,000 cells can be cryopreserved.

The culture room

four clean benches and one safety cabinet.

four large incubators and four small incubators (for hypoxic culture).

Med64

Easily measure the electrical activity of neural cells and skeletal muscle. Used in combination with the optogenetics system.

Right:Inverted fluorescence microscope (OLYMPUS IX83) in Darkroom

The UPLSAPO super apochromat objectives are installed. The camera ORCA-Flash4.0 serves refined wider views.

Left:Upright microscope (Zeiss)

Confocal laser scanning microscope

Can take high-resolution and appealing pictures with simple operations.
Used for observing cell dynamics with time-lapse imaging.

Upper: Fluorescence microscope

Used for observing fluorescently labeled cells transplanted into mice.

Bottom:UV transilluminator

Used for gel imaging and band cutting.。

IN Cell Analyzer 6000 (Common use in the university)

An imaging cytometer that can acquire confocal images at high speed and enables quantitative analysis.
Used for time-lapse photography to analyze neurite outgrowth of iPSC-derived motor neurons.

PC for analyzing data of IN Cell Analyzer

Developper Toolbox and IN Carta are installed.